We will now take an individual look at the prospects of Plymouth Argyle, Exeter City and Torquay United.
CHELTENHAM TOWN VS PLYMOUTH ARGYLE
The Pilgrims travel to Whaddon Road in what will be manager John Sheridan's first match in the competition since he took charge at Home Park.
Sheridan has won the Trophy before, in 2012, whilst he was in charge of Chesterfield when the Spireites defeated Paolo Di Canio's Swindon Town 1-0 in the final at Wembley.
He stated after the draw was made that he would be taking the competition 'very seriously' as it was a tournament that the Pilgrims stood a realistic chance of winning.
It will also be the second time that Argyle have faced the Robins in the competition. Their first meeting came in the 2001-02 season, when Argyle lost 2-1 after taking the lead through David Friio.
The second came in the 2010-11 campaign, under Peter Reid, when the Pilgrims won 2-0 with Steven MacLean and Craig Noone finding the target.
EXETER CITY VS WYCOMBE WANDERERS
Exeter City's record in the Johnstone's Paint Trophy has seen them reach the Southern Area Final three times (1993, 2000 & 2011) but they have never reached the final. Another similar run in the competition this year would come as a timely boost to the club's finances which would, along with the windfall they recently received from Scott Golbourne's transfer to Wolves, possibly give manager Paul Tisdale extra room for manoeuvre in terms of squad strengthening.
The prospect of the Grecians making initial progress in the competition this term look particularly good as they currently have a 100% home record from their three home games this season.
TORQUAY UNITED VS PORTSMOUTH
In 2008, Torquay United and Portsmouth both played at Wembley, the Gulls
contested the FA Trophy final, which they lost to Ebbsfleet United, whilst
Portsmouth won the FA Cup against Cardiff City. The Gulls famously reached the final of the competition, under one of it's many previous guises as the Sherpa Van Trophy, in 1989 when they lost to Bolton Wanderers, after shocking Wolves in the Southern Area final second leg at Molineux, and would surely love to do so again.
It will also provide manager Alan Knill with a dress rehearsal for their league meeting at Plainmoor, which is due to take place at the end of October.
For Pompey a good run in the competition could help with their long term revival, as they aim to join the list of previous winners that includes Wolves, Birmingham City, Wigan Athletic as well as their fierce local rivals Southampton.
Footnote: When Wolverhampton Wanderers enter the competition at the next stage, it will be the first time that they would have played in the competition since their Southern Area final defeat at the hands of the Gulls at Molineux in 1989.
